POSSIBILITIES OF USING ALLELIC POLYMORPHISM OF INTERLEUKIN-6 G-174C AND TUMOR NECROSIS FACTOR-Α G308A GENES FOR PREDICTION OF JUVENILE IDIOPATHIC ARTHRITIS COURSE

Oshlyanska O.A.¹, Artsymovych A.G.¹, Rossokha Z.I.², Okhotnikova O.M.¹, Popova O.F.², Medvedeva N.L.², Vershigora V.O.², Chaikovskyi I.A.³, Krivova O.A.⁴

• ¹National University of Health of Ukraine named after P.L. Shupika
• ²SU «Reference Center for Molecular Diagnostics of the Ministry of Health of Ukraine»
• ³VM Glushkov Institute of Cybernetics of the National Academy of Sciences of Ukraine
• ⁴International Research and Training Center for Information Technologies and Systems of the National Academy of Sciences of Ukraine and the Ministry of Education and Science of Ukraine

Summary. Juvenile idiopathic arthritis is a disease whose pathogenesis is based on an imbalance of pro- and anti-inflammatory cytokines; therefore, the study of the cytokine profile is widely used when working with patients with JIA. But the determination of the level of cytokines in the peripheral blood can only help in assessing the current state of the patient and the choice of therapy. Allelic polymorphism of genes encoding proinflammatory cytokines has been studied for a long time as a possible tool for long-term prediction. Purpose: to study allelic polymorphism of genes for interleukin-6 G174C and tumor necrosis factor-α G308A in children with JIA, depending on the characteristics of its course. Subject: Pediatric patients with a pre-established diagnosis of JIA. Materials and methods: clinical, instrumental and laboratory examination of 52 patients aged 2 to 17 years, who were treated at the clinical base of the Department of Pediatrics No. 1 of the Shupyk National University of Healthcare of Ukraine, pediatric department of SI «IPOG of NAMS of Ukraine named after academician O.M. Lukyanova». Additionally, all patients underwent determination of allelic polymorphism of genes G174C and G308A. Results: according to the obtained variants of polymorphism, the patients were divided into 7 groups. Each of the described groups had separate phenotypic features, such as the presence of pathognomonic antibodies, the presence or absence of extra-articular lesions, a more or less high frequency of cases with an unfavorable course. In groups with mutant alleles, the course of the disease was usually less favorable; such patients more often required correction or escalation of therapy. Conclusions: further study of the allelic polymorphism of the interleukin-6 G174C and tumor necrosis factor-α G308A genes in children with JIA may be promising for predicting the course of JIA in patients.