THE ULTRASTRUCTURAL FEATURES OF ALVEOLOCYTES OF TYPES I AND II IN EXPERIMENTAL MODELING OF SYSTEMIC SCLEROSIS AND IT’S CORRECTION
Summary. Aim. To test the hypothesis about the possible effectiveness of vitamin D3 (vit. D) and alpha-tocopherol acetate (vit. E) on the morphological structure of type I (AI) and II (AII) alveolocytes. Materials and methods. Laboratory animals of the Wistar line 220-240 g were divided into four groups: intact (IG), control (CG), experimental group 1 (EG1) and experimental group 2 (EG2). EG1 animals were injected with NaClO in a dose of 0.5 ml of a 5% solution three times a week subcutaneously for 6 consecutive weeks. In EG2, an intramuscular injection of vitamin D (1000 IU/100 g) and vitamin E (10 mg/100 g) was in addition performed intramuscularly. Results. The conducted histological and electron microscopic study showed that the introduction of vit D and vit E has a positive effect on the structure of lung alveolocytes, reducing pathological changes, such as aggregation of chromatin granules, destruction of mitochondria and ruptures of the plasma membrane. Morover, the use of these drugs helped reduce the severity of edematous changes in cellular structures of type AI and AII. An improvement in the structural characteristics of lamellar bodies was also observed in AII type. Conclusion. The obtained results indicate the potential benefit of using vitamin D3 and alpha-tocopherol acetate in the correction of respiratory lesions associated with systemic sclerosis.
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