INFLUENCE OF LIPOPOLYSACCHARIDE AND DEXAMETHASONE ON PROPERTIES OF ADIPOSE TISSUE MULTIPOTENT STROMAL CELLS OF PATIENTS WITH SYSTEMIC LUPUS ERYTHEMATOSUS

Nikolsky I.S.¹, Nikolska V.V. ¹, Protsenko G.O.¹, Podpryatov S.E.², Podpryatov S.S.², Semenova Ya.-M.O. ¹, Taranukha L.I.¹

• ¹State University «National Scientific Center «Institute of Cardiology, Clinical and Regenerative Medicine named after Academician M.D. Strazhesko, National Academy of Medical Sciences of Ukraine», Kyiv
• ²Kyiv City Clinical Hospital No. 1, Kyiv

Summary. Systemic lupus erythematosus (SLE) is an autoimmune disease, and therefore, perhaps, the most adequate approach to the development of more effective treatment methods is to improve methods of regulating the activity of the immune system. In this aspect, cell therapy is considered a promising direction, one of the effective means of which is multipotent stromal cells (MSCs). A large number of works in the experiment and clinic are devoted to MSCs of adipose tissue (AT MSCs), which can be explained by the relatively simple method of obtaining them and their known immunoactive capabilities. In order to improve the cultural and transplantation properties, studies on the modification of MSCs are being conducted. Biologically active substances are often used for this in vitro. The paper presents the results of the influence of lipopolysaccharide (LPS) and dexamethasone, one of the most effective immunoactive substances, on the cultural properties of MSCs. Aim. To study some of the cultural properties of AT MSCs of patients SLE and to study the possibilities of a positive effect on them of immunoactive factors: pro-inflammatory lipopolysaccharide and anti-inflammatory dexamethasone and in general to assess the prospects of developing cell therapy methods using these factors. Materials and methods. AT MSCs of patients with SLE and cosmetology patients, in which cells were obtained from excess adipose tissue (comparison group), were studied. It was carried out cultivation of AT MSCs, determination of parameters of cell population growth kinetics, study of colony-forming units of fibroblasts (CFU-F), study of contact interaction of AT MSCs with lymphocytes and results of cryopreservation of MSCs. The results. Adapted methodical approaches to obtaining cultures of AT MSCs of patients with SLE. It was established that the kinetics of growth of AT MSCs of patients with SLE compared to that of AT MSCs of cosmetology patients is significantly slowed down, and the ability of contact interaction of AT MSCs with thymocytes and the efficiency of cloning of AT MSCs of patients with SLE is significantly reduced. Under the influence of LPS, the growth kinetics of AT MSCs in patients with SLE is significantly stimulated, dexamethasone does not affect the growth kinetics, but has the ability to cancel the stimulating effect of LPS. In the same way, the effect of LPS and dexamethasone on the ability of contact interaction of MSCs with thymocytes and the efficiency of AT MSC cloning is realized. Cryopreservation has practically no effect on the viability of AT MSCs. Conclusions. Adapted methodical approaches to obtaining LPS-stimulated AT MSC cultures of patients with SLE. It was established that the growth kinetics of AT MSCs of patients with SLE is significantly slowed down. the ability of AT MSCs to interact with thymocytes and the cloning efficiency of AT MSCs from patients with SLE is also significantly reduced. Under the influence of LPS, the growth kinetics of AT MSCs in patients with SLE is significantly stimulated, dexamethasone does not affect the growth kinetics, but has the ability to cancel the effect of LPS. In the same way, the effect of LPS and dexamethasone on the ability of MSCs to interact with thymocytes and the efficiency of AT MSC cloning is realized. Cryopreservation practically does not affect the viability of AT MSCs.